by determination of optical density by coloimetric method
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17 Bio Technology Questions And Answers
Glucose is reactive because its open-chain form contains an
1- restriction enzyme
2- ligase enzyme
3- formation of recombintant plasmid by using these enzyme
4- selection of transformed cell
5- cDNA and genomic library
The father of term biotechnology is Karl Ereky and father of
biotechnology is Louis Pasteur
PhD is a threshold qualification about research, & makes capable to present your idea/view as a Philosopher.
By genetically modifying any plant such that it will resist
the climate in dubai n still will give fruits n green
may be cutting a gene code from cactus plant and pasting it
in apple plant...
1- In drug discovery research
2- Analysis of genome wide biomedical data
3- In genome and sequence analysis
define serum is the artificial serum made by man and
composition of which is well known by us
characterized serum is that which made up of specific
characters for example it contains specific antibody against
stander serum is serum which is collected from normal blood
which we can not make in laboratory scale although we know
composition of it.
Why, bacteria eat almost anything they can get! Of course,
they can't chew through cement. They can eat anything
edible to animals and humans, probably more! Take wood for
example, termites eat wood, bacteria eat wood too. In fact,
termites wouldn't even be able to eat wood if it wasn't for
the bacteria in their stomachs!
Bacteria are decomposers. Decomposers eat anything dead and
once they are finished they put nutrients from the dead
object or plant back into the ground to grow new plants
Transfection:Transfer of naked dna into eukaryotic cell
Transduction:Trnsfer of genetic material from bacteria to
virus through bacteriophage
12⟩ DOES EUKARYOTES HAVE RESTRICTION MODIFICATION SYSTEM? IF YES, THAN WHY ARE WE NOT USING FOR THE TREATMENT OF HIV?
eukaryotes does not contain restriction modificayion system
13⟩ What is the difference between transfection and transduction?why the word trasfection is prefers for transfer of genetic material through non viral particles in eukaryote not for prokaryote?
Transfection- (Transfer+ Infection)transfer of naked DNA
(DNA without any carrier)into an Eukaryotic cell.
Transduction-Transfer of genetic material among bacteria by
Since the term 'Transformation' is used in case of transfer
of naked DNA (through non viral particles) into
prokaryotes,while the term 'Transfection' is uused in case
of eukaryotes because the term transformation is used for
the conversion of normal body cells into cancerous cells
which undergo unlimited cell division, so to avoid the
confusion between these two different cell processes the
other term Transfection is used in eukaryotes in place of
the term transformation in prokaryotes.
it is used to determine the protein from a mixture of compounds
15⟩ Why do you use DBM instead of NCF in the northern blotting and what are the pre-treatments required for this paper to be used?
diazobenzyloxymethyl paper that binds all single-stranded DNA, RNA, and proteins by means of covalent linkages to the diazonium group; used in situations where nitrocellulose blotting is not technically feasible
positively charged i.e,cationic nature
rna is having _ve charge & DBM paper having positive chrg
hence due to thease opposite charges DBM paper binds to RNA