I) If transformant is thought to have antibiotic resistant

gene (called as Marker Gene)along with ur desired gene, then

culture those cells in a media containing that same

antibiotic.

==>Case 1:- the cell is not transformed.:-

the cell will not have the antibiotic resistant gene, hence

it wont grow (or simply die)bcoz of antibiotic in the media.

==>Case 2:-the cell is successfully transformed.:-

The cell will easily grow in media bcoz antibiotic wont

affect it as cell will resist it.(bcoz of marker gene).