Mainly water and light, but also mineral salts, temperature, and carbon dioxide are factors that interfere with the gross primary productivity.
Phase Contrast microscopy A) Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen. B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices. C) Uses special lenses to distinguish between solid and liquid phases of the cell. D) Uses special lenses to change the color of light passing through them
Which of the following statements about Transmission Electron Microscopy is not true. A) The specimen must be stained with osmium or other heavy metal. B) The specimens are placed in a high vacuum for viewing. C) The specimens must be sliced very thin, 20-100 nm in thickness. D) The beam is focused by electromagnetic lenses
Which of the following statements is most correct about Atomic Force Microscopy (AFM)? A) AFM can visualize protein bound to DNA molecules. B) AFM can visualize unfixed specimens in water or buffer. C) AFM moves a very sharp tip over the surface of the specimen to "feel" its shape. D) All the statements above are true
Which of the following statements is most correct about the differential Gram stain? A) Crystal violet differentially stains Gram positive cells. B) Gram's iodine differentially stains Gram positive cells. C) Acetone differentially destains Gram negative cells. D) Saffron red differentially stains Gram negative cells
Which of the following objectives would give you the best resolution of small objects? A) 10x air, N.A. 0.25 B) 40x air, N.A. 0.65 C) 64x oil, N.A. 1.4 D) 100x oil, N.A. 1.25
Transmission electron microscopy is best for high magnification viewing of A) internal structure of fixed cells. B) internal structure of live, motile cells. C) surface structure of fixed cells. D) surface membranes of live, motile cells
Monochromatic (one color) light is sometimes used to increase the resolution of light microscopes. Light of which color below would give you the best resolution? A) Red B) Orange C) Green D) Blue
Scanning electron microscopy (SEM) is best used to study A) small internal cell structures. B) surface morphology. C) all of the above
Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens? A) 0.2 microns B) 0.3 microns C) 0.9 microns D) 0.25
A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a A) transmission electron microscope B) scanning electron microscope C) phase-contrast microscope
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