Step 1. Assemble your sequences.

Put all your sequences in Fasta format in a single file.

This file should be located in a suitably named

subdirectory of your home directory on the UBiC Blast

server. The definition line for each sequence should start

with a unique identifier for that sequence.

Step 2. Convert this sequence file to a Blastable database.

The command formatdb converts your Fasta file of sequences

to a set of files that can be queried with command-line

BLAST.

Step 3. Test Blast on your database.

See the UBiC tutorial, Using Command-line BLAST. In the

blastall command-line you will need to specify the location

of your database by typing: -

d /disk2/home/myhome/blastdbs/custom.aa.