Although your site may be handling x number of users per day, only a small percentage of these users would be hitting your site at the same time. For example, if you have 3000 unique users hitting your site on one day, all 3000 are not going to be using the site between 11.01 and 11.05 am.

So, once you have identified your peak hour, divide this hour into 5 or 10 minute slices [you should use your own judgement here, based on the length of the average user session] to get the number of concurrent users for that time slice.