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“Biomedical engineer Frequently Asked Questions in various Biomedical Engineer job interviews by interviewer. The set of questions are here to ensures that you offer a perfect answer posed to you. So get preparation for your new job interview”



132 Biomedical Engineer Questions And Answers

82⟩ What is microarrays. How are they related to DNA?

Microarrays are matrix in the form of arrays where DNA oligonucleotides of DNA sequences are spotted. They can be used for gene expression profiling, single nucleotide polymorphism detection, detection of alternative splicing and for various other purposes. Microarrays have the capability to perform hybridization of coda with the help of probes. A microarray chip is capable to perform a large set of genetic related experiments concurrently.

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83⟩ Tell me what is therapeutic cloning?

In the process of cloning DNA or a part of DNA is duplicated. This process is also called somatic cell nuclear transfer. In this process embryo is utilized for cloning. Embryo comprises of stem cells which is later employed in regeneration applications. The embryonic stem cells are renewable and are pluripotent.

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84⟩ What is myoelectric control?

Myoelectric control uses the signals from a residual limb for the movement of the prosthetics. Myoelectric control technologies obtain signals from the skin on the limbs.

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86⟩ Explain the difference between retrovirus and provirus?

A retrovirus is a RNA virus which can be duplicated in a host cell using the reverse transcriptase enzyme. It can produce DNA from its RNA genome. The produced DNA is then incorporated into the host's genome by anintegrase enzyme. The RNA virus thereafter replicates as part of the host cell's DNA. Retroviruses are enveloped viruses that belong to the viral family Retroviridae.

Provirus is a virus genome which can integrate into DNA of host cell. In inactive viral infections the virus will not replicate itself but through replication of its host cell. This state can last over many host cell generations.

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87⟩ What is a microarray?

Microarrays are arrays where DNA oligonucleotides of DNA sequences are spotted as a matrix. Microarrays are used in gene expression profiling, single nucleotide polymorphism detection, detection of alternative splicing etc. Microarrays perform hybridization of cDNA using probes. A microarray chip has the capability to perform a large set of genetic related experiments simultaneously.

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90⟩ Fresh Biomedical Engineer Interview Questions

☛ What si the self antigen for scleroderma?

☛ What is allograft?

☛ what is xenograft?

☛ What are intereferons?

☛ What is an effector cell?

☛ What is an effector response?

☛ Name the target antigen for T cell anemia.

☛ What are exogenous antigens?

☛ What is an agglutinin?

☛ Why is buprenorphine less addictive than other opioids?

☛ Which type of immunoglobin level will increase when an individual is exposed to parasite?

☛ Where do most allergic reactions occur?

☛ What is an atopy?

☛ Who are atopic individuals?

☛ Explain blood brain barrier.

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91⟩ What is prosthetic limb? What are its drawbacks?

It is an artificial device which can be used to replace a missing body part. It is based on the principle of biomechatronics. It can be used to replace body part missing from birth, due to injury or due to defect. The main drawback of prosthetic limb is its cost. Moreover, prosthetic limbs have to be replaced every 3-4 year due to the wear and tear. If the limb has fit tissues then the sockets in the limb have to be replaced every month.

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92⟩ What is superiority of TLC over paper chromatography?

TLC is superior over paper chromatography because of inorganic nature of adsorbent concentrated sulfuric acid spray. The spray is then followed by heating. It may be used to develop on the chromatogram by charring. Also, amino acid mixtures require 18 hours for separation on paper. It requires 3 hrs using cellulose TLC. The advantages of TLC lie in adsorbents which don't allow separation on paper. In TLC we have much wider choice of adsorbents depending upon needs and sample.

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93⟩ What is isotopic tracer technique?

This technique is used to understand chemical reaction and interactions in bio-chemistry and chemistry. In this technique, one or more of the atoms of the molecule of interest is substituted for an atom of the same chemical element, but that element belongs to different isotope. It can be used to detect the difference in number of neutrons separately from the other atoms of the same element. The atom has the same number of protons; it will behave in almost exactly the same way chemically as other atoms in the compound, and with few exceptions will not interfere with the reaction under investigation.

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94⟩ Explain the mechanism of ELISA. What are its uses?

ELISA stands for enzyme linked immunosorbent assay. It is a wet lab type analytical biochemistry assay. It can detect presence of a substance in a liquid or wet sample by using one subtype of heterogeneous, solid-phase enzyme immunoassay. ELISA can be used as ligand binding assays. It is also used as a diagnostic tool in medicine and plant pathology. Quality check operations can also be performed in various industries with the help of ELISA.

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95⟩ Tell me what is Alzheimer's disease?

Alzheimer's is a brain disease caused due to tau protein misfolding. It is an incurable disease and can be diagnosed in a PET or MRI scan. Alzheimer's is related more with aging, where the disease is detected in human more than 65 years of age. The symptoms are memory losses, stress, confusion and also aggression. Diagnosis is mostly done by behavior related tests.

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96⟩ What are immunoglobulins? Explain its structure?

Immunoglobulins are popularly known as antibody. These are large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. Immunoglobulins are "Y" shaped structure which is having two tips and each tip of immunoglobulins contains a paratope. Immunoglobulins are typically made of basic structural units-each with two large heavy chains and two small light chains. The general structure of all antibodies is very similar; a small region at the tip of the protein is extremely variable.

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98⟩ What is pathogens. Name some types of pathogens?

Pathogens are those organism which feeds on other organism for their food. Pathogens can be transported through many different routes, including airborne, direct or indirect contact, sexual contact, through blood, breast milk, or other body fluids, and through the fecal-oral route. Pathogens can be used to suppress pest population. Different types of pathogens are viral, bacterial, fungal etc.

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99⟩ Explain what is the principle behind DNA fingerprinting?

DNA fingerprinting is the technique of genetic fingerprinting. In this technique, DNA sequence can be used for identification of an individual. The main application of DNA fingerprinting is forensics. The main principle behind behind DNA fingerprinting is Polymerase Chain Reaction. This technique is also popularly known as DNA profiling.

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100⟩ Basic Biomedical Engineer Job Interview Questions

☛ What is the treatment of HIV?

☛ What are the results of HIV?

☛ What is the current treatment given to AIDS?

☛ What does immunodeficiency results?

☛ What does myeloid immunodeficiency cause?

☛ How viral load can be measured?

☛ What is an abzyme?

☛ What is adoptive transfer?

☛ What is apoptosis?

☛ What is an agretope?

☛ What is a booster?

☛ What is bispecific antibody?

☛ What is antigenic competition?

☛ What is autograft?

☛ What is the self antigen for haemolytic anemia?

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