Analytical Chemistry

pH OF THE BUFFER SOLUTION WILL BE CHANGE, AS DUE TO PRESENCE OF NITROGEN CONTAINING SUBSTANCES AND ALSO DUE TO MICROBIAL GROWTH. BUT IF STORED AT 4 DEGREE CENTI. IT MAY USED TO SOME EXTENT.

IF ALL IMPURTITIES AT SPEC LEVEL ARE MEETING S/N RATIO MORE

THAN 60 AT THAT PARTICULAR CONCENTRATION AS WEL AS NO

COLUMN OVERLOAD.

Reference std is like USP,EP std , in this std we get COA

including results of Potency by HPLC , NMR & XRPD data.

whenever there is requirement of w.std preparation first we

have get API rawmaterial from warehouse & we check assay ,

water content or LOD & Related substances aganist reference

std . Some times we are doing analysis in duplicate & get

mean value.then we decide final assay or potency value &

it's validity one year

Isotopes of H2O, D2O

quality control means to maintain the quality of product by

calculating their content ,different physical parameters,as

per their specification IP/BP/USP/EP/JP

ACN is highly polar as compare to Methanol So provide Better

resolution for many compound and it has property to form

hydrogen bond so provide better selectivity.

Check for the number of plates that it maintains stady all

over the run , check the SST at avery run that cpmplies the

acceptance criterias of the method.

RS stands for related substance, means by-products upon

completion of reaction, or the unreacted portion present in

the product. RS testing help us to identify the adequate

quantitiy of these by-products or unreacted part.

More over RS help us during the stability study to know any

significant change in the nature of product during long

storage in various climatic conditions

Actually RRF establishment required For Relatives Substances

Why because in Rs Sample having so many components like impurities,Degradents and main Analytes etc..so all components does not have same wavelength but we are fix one particular wavelength for all peaks. so inthat wavelength some peak response high and some peak response low.so that reason only we are established RRF and impurities also expensive so every time we can't purchase.so once established the RRF that factor used for RS calculation

it depend on the solubility of analyte whether it is soluble in polar or non polar solvent apart from that retention time, capacity factor to prepare suitable mobile phase.

That sample converted chromofore by derivitisation technique and going to analysis

Tlc is called thin layer chromatography. The simple technique works on the principle of adsorption, where as HPLC is a sophisticated technique works on partition principle.

5 mg of water consuming 1 ml of KF reagent.

Just you want to know about that please just refer your SOP (STANDARD OPERATING PROCEDURE)

I DO NOT KNOW

C8 column have more polar than C18. and it has 8 carbon atoms.

Purity is only area normalization.

Rs is calculated by using std and rrf

nitrogen

When analyte gives linear response then only we can use area normalization. When analyte response is to high and saturated in chromatograph, for this condition we can use diluted standard.

NO