21⟩ Negative stains color the structures of interest a deep black. A) True B) False
B) False
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50 Electron Microscopist Questions And Answers
22⟩ Bacterial flagella are too small to be seen by light microscopy without staining the specimen. A) True B) False
A) True
23⟩ Transmission electron microscopy has a practical magnification of greater than 100,000x. A) True B) False
A) True
24⟩ Chromogenic stains which consist of a single dye rather than several dyes in combination are known as basic stains . A) True B) False
B) False
25⟩ Acid fast stains interact with the waxy structures of the cell wall of Mycobacterium species. A) True B) False
A) True
26⟩ Fluorescence microscopy may be used with specially stained antibodies to detect specific microorganisms. A) True B) False
A) True
27⟩ Fluorescence microscopy must be done on fixed and stained microorganisms. A) True B) False
B) False
28⟩ Darkfield microscopy is best for visualizing live, motile microorganisms. A) True B) False
A) True
29⟩ Objective lenses with higher magnification generally have a shorter working distance. A) True B) False
A) True
30⟩ The fluorescence microscope has become an essential tool in medical microbiology to identify bacterial pathogens. A) True B) False
A) True
31⟩ Microscope resolution increases as the wavelength of radiation used to illuminate the specimen decreases. A) True B) False
A) True
32⟩ Even on the best light microscopes, increasing total magnification beyond 1500 cannot result in better resolution of the image. A) True B) False
A) True
33⟩ Differential Interference Contrast microscopy A) compares two identical specimens on the same microscope. B) illuminates the specimen with light of two different colors. C) illuminates the specimen with light of two different phases. D) illuminates the specimen with both reflected and transmitted light
C) illuminates the specimen with light of two different phases.
34⟩ In a compound microscope, the primary image is formed by an objective lens and enlarged by the optical lens to reveal a virtual image. A) True B) False
A) True
35⟩ "Parfocal" refers to microscopes with multiple objectives where A) objectives are used in pairs for stereoscopic effects. B) each objective has the same working distance above the specimen. C) each objective is positioned to be in focus at the same stage height. D) sequential objectives increase power by a factor of two.
C) each objective is positioned to be in focus at the same stage height.
36⟩ Which of the following is NOT equivalent to 10 micrometers. A) 0.0001 cm B) 0.01 mm C) 10,000 nm D) 100,000 Angstroms
A) 0.0001 cm
37⟩ Phase Contrast microscopy A) Continuously changes the phase of the incident light from the condenser to improve contrast in the specimen. B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices. C) Uses special lenses to distinguish between solid and liquid phases of the cell. D) Uses special lenses to change the color of light passing through them
B) Uses circular filters in the condenser and objective to give contrast to parts of the cell with different refractive indices.
38⟩ Which of the following statements about Transmission Electron Microscopy is not true. A) The specimen must be stained with osmium or other heavy metal. B) The specimens are placed in a high vacuum for viewing. C) The specimens must be sliced very thin, 20-100 nm in thickness. D) The beam is focused by electromagnetic lenses
A) The specimen must be stained with osmium or other heavy metal.
39⟩ Which of the following statements is most correct about Atomic Force Microscopy (AFM)? A) AFM can visualize protein bound to DNA molecules. B) AFM can visualize unfixed specimens in water or buffer. C) AFM moves a very sharp tip over the surface of the specimen to "feel" its shape. D) All the statements above are true
D) All the statements above are true
40⟩ Which of the following statements is most correct about the differential Gram stain? A) Crystal violet differentially stains Gram positive cells. B) Gram's iodine differentially stains Gram positive cells. C) Acetone differentially destains Gram negative cells. D) Saffron red differentially stains Gram negative cells
C) Acetone differentially destains Gram negative cells.
