41⟩ Which of the following objectives would give you the best resolution of small objects? A) 10x air, N.A. 0.25 B) 40x air, N.A. 0.65 C) 64x oil, N.A. 1.4 D) 100x oil, N.A. 1.25
C) 64x oil, N.A. 1.4
Electron Microscopist
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50 Electron Microscopist Questions And Answers
42⟩ Transmission electron microscopy is best for high magnification viewing of A) internal structure of fixed cells. B) internal structure of live, motile cells. C) surface structure of fixed cells. D) surface membranes of live, motile cells
A) internal structure of fixed cells.
43⟩ Monochromatic (one color) light is sometimes used to increase the resolution of light microscopes. Light of which color below would give you the best resolution? A) Red B) Orange C) Green D) Blue
D) Blue
44⟩ Scanning electron microscopy (SEM) is best used to study A) small internal cell structures. B) surface morphology. C) all of the above
B) surface morphology.
45⟩ Resolution is the ability of a lens to distinguish between small objects close together. What approximate resolution can be obtained with a lower power (10X, N.A. 0.25) objective lens? A) 0.2 microns B) 0.3 microns C) 0.9 microns D) 0.25
C) 0.9 microns
46⟩ A microscope in which an image is formed by passing an electron beam through a specimen and focusing the scattered electrons with magnetic lenses is called a A) transmission electron microscope B) scanning electron microscope C) phase-contrast microscope
A) transmission electron microscope
47⟩ Living, unstained cells and organisms can be observed best using A) fluorescent microscopy B) TEM C) phase contrast microscopy
C) phase contrast microscopy
48⟩ Fixation is the process by which the structures of the cells are preserved and fixed in position. An advantage of chemical fixation over heat fixation is that it A) magnifies the specimen B) does not destroy internal structures
B) does not destroy internal structures
49⟩ The substage condenser A) changes the wavelength of the light reaching the specimen B) focuses light on the specimen C) decreases the amount of light reaching the specimen
B) focuses light on the specimen
50⟩ A 40X objective and an 10X ocular produce a total magnification of A) 50 B) 400 C) 90
B) 400
